Rapid Test Pro

Q&A regarding Rapid Test Pro

Questions regarding performance

Q1. What can be detected by Rapid Test Pro?
A1. Proteins derived from egg, milk, wheat, buckwheat and peanut can be detected.
Q2. How long does an assay take?
A2. It takes 30-60 minutes from the preparation to the determination.
It is also possible to assay easier, depending on the subject and the sample.
Q3. Does the kit contain any reagents regulated under Poisonous and Deleterious Substances Control Law and/or PRTR?
A3. No reagents regulated under the laws are contained.
Q4. What is the sensitivity of the kit?
A4. The kit shows positive when 25 ng/mL or more of total protein of specific raw material is in the Test Solution, which corresponds to 5 μg/g or more if converting into that of food.
Q5. Can you tell me about cross-reactivity to food materials?
A5. Please refer here.
Q6. Can you tell me about false-negative samples?
A6. As the reactivity between antigen and antibody in protein hydrolysates and similar products changes, the assay may give underestimates rather than exact amounts.

Questions of reagents and handling

Q7. Is it possible to use the sample extraction solution of Rapid Test Pro in the series of Rapid Test Pro in common?
A7. Yes. Extraction Solution can be used commonly in the series of Rapid Test Pro.
Q8. Is it possible to use dilute solution of Rapid Test Pro in the series of Rapid Test Pro in common?
A8. Yes. Diluent① and Diluent② can be used commonly in the series of Rapid Test Pro.
Q9. Can I use the extracted solution prepared with Food Allergen ELISA Kit or ELISA Kit II series?
A9. For Food Allergen ELISA Kit II series – Yes, the composition of the extracted solution with Rapid Test Pro is the same.
For Food Allergen ELISA Kit series – No, the composition of the extracted solution with Rapid Test Pro is different.
Q10. After opening the reagents, can I keep them for later use?
A10. Although Diluent① and Diluent② can be used until the expiration date described in the kit after opening them, please handle carefully enough not to contaminate the solutions with another reagent at their opening and storage.
Test sticks are packed individually, so they can be opened just before use.
Q11. Can I keep the prepared sample extraction solution?
A11. Yes, the Extraction Solution of Rapid Test Pro series is ready to use and can be kept until the expiration date.
Q12. What can I do in cases of using less than 1 g of the sample? (in case of food inspection)
A12. In cases of using less than 1 g of the sample, extraction with 19 mL of Extraction Solution may lead to false negatives because of decreasing the antigen. Thus, in these cases, adjust the Extraction Solution volume appropriately so that the ratio of the sample and the Extraction Solution is 1:19.
Q13. Can I execute a swab test with a swab moistened with saline?
A13. Yes. But, it is impossible to test by adding saline to the test stick directly.
Q14. On heating, is it better to loosen the lid of the tube?
A14. No. Be sure to tighten the lid of the tube, to avoid contamination during heating.
Q15. For the Recommended Extraction Method, can I shorten the 10-minute heating period by incubating the tube in boiling water?
A15. No. To obtain correct test results, please execute 10-minute heating in boiling water.
Q16. Are there any problems when the heating time in boiling water is over 10 minutes?
A16. No. To obtain steady test data, the heating time should be no less than 10 minutes.
Q17. How can I cool the reaction mixture in a tube?
A17. Immerse the tube in running water or a water bath or in a similar way to cool it slightly.
Q18. (In case of food inspection,) how can I handle samples for dilution followed by examination?
A18. After the Sample Extract is diluted with Extraction Solution appropriately, dilute them 10-fold with Diluting Solution for examination.
Q19. (In case of swab test,) how can I perform dilution followed by examination?
A19. After the Sample Extract is diluted with Extraction Solution appropriately, dilute them 10-fold with Diluting Solution for examination.
Q20. Can I assay the Test Solution diluted with Diluting Solution in different ratios than 10-fold?
A20. No. You must use Test Solution 10-fold diluted with Diluting Solution.
Q21. The Confirmation window on the test stick removed from the pillow case is colored black. Is this possible to use?
A21. There are no problems with the product capability. A reagent is smeared on the confirmation window, so that in some cases it turns black.
Q22. I opened a package and left it. Can I use it?
A22. Once a pillow case is opened, the test stick absorbs moisture so that depending on cases it affects the development of an assay solution. Accordingly, please avoid using the test stick after the time has passed since the pillow case is opened. Open a pillow case just before use of the test stick and use it immediately after opening.
Q23. How should I treat the test stick during reaction?
A23. Because of the possibility to affect the development of the assay solution, moving the test stick in reaction should be avoided. Place the test stick in reaction on a horizontal plane.
Q24. Any concerns for handling the test stick?
A24. Be sure to turn the test stick to room temperature (20-30 °C) before opening, and use immediately after opening. In addition, please make sure not to touch the sample application slot and the test window when you remove the test stick from a pillow case.

Questions of determination

Q25. I used a test stick without turning it to room temperature. Are there any effects on the result?
A25. Test sticks should be turned to room temperature (20-30 °C) before opening and the use. When you open a pillow case with lower temperature, the test stick absorbs moisture and it is hard to make the determination correctly in some cases. Furthermore, when the temperature during the reaction is lower than room temperature, the antigen-antibody reaction may be affected and in some cases the appearance of the line that shows the sample is positive may be delayed, so please take care regarding the reaction temperature.
Q26. The test window as a whole in reaction is colored red. Are there no problems?
A26. There are no problems even the test window as a whole is colored red in reaction. This is on a reaction process where assay solution and reagents are flowing. Do not make determination at this time. Leave as it goes and be sure to make determination after the specific reaction time.
Q27. After adding of the Test Solution, can I make determination based off a line on the test window before the end of the specific reaction time?
A27. No, Rapid Test Pro is designed to make determination following the end of the specific reaction time of 15 minutes (for Rapid Test Pro for Buckwheat, 25 minutes). Although there are some cases of seeing a line and colored confirmation window, please be sure to check result when that the specific reaction time has passed.
Q28. Can I make a determination based off the information taken after the reaction time has passed?
A28. No, Rapid Test Pro is designed to make a determination after the reaction time of 15 minutes (for Rapid Test Pro for Buckwheat, 25 minutes). Correct determination results are difficult to be obtained when the condition is over the reaction time. Please be sure to check result at the time that the specific reaction time has passed.
Q29. The determination at the end of the reaction time was negative. Some time after that, a line appeared on the test window. How can I make a determination about this?
A29. Rapid Test Pro is designed to make determination after the reaction time of 15 minutes (for Rapid Test Pro for buckwheat, 25 minutes). Because the result may change over time depending on samples, please be sure that the result at the end of the reaction time is taken. Even when seeing a line on the test window over the time, the result was negative when the reaction time had passed, so please handle the determination result as negative.
Q30. Are there any factors to affect the assay result?
A30. Two factors as follows are possible to affect the assay result adversely:
extreme acidity and basicity, ionicity (strength and type), viscosity, protein hydrolase, surfactant, oxidation, alcohol and similar agents may affect the assay result;
a highly colorable sample may be difficult to validate a clear band.

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